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transit oligo transfection reagent  (Mirus Bio)


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    Structured Review

    Mirus Bio transit oligo transfection reagent
    Transit Oligo Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transit+oligo+transfection+reagent/TransIT+-Oligo/pm41167556-247-19-22
    Average 93 stars, based on 51 article reviews
    transit oligo transfection reagent - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Hydrogel-Assisted Antisense LNA Gapmer Delivery for In Situ Gene Silencing in Spinal Cord Injury
    Article Snippet: .. Transfections were conducted using the TransIT-Oligo Transfection Reagent (MirusBio). .. Briefly, 4 μL TransIT reagent was mixed with 50 μL serum-free Opti-MEM (GIBCO), after which 4.5 μL AON (20 μM) was added.

    Article Title: RNA Structural Ensemble Determinants of -1 Programmed Ribosomal Frameshifting Efficiency Across Coronavirus Evolution.
    Article Snippet: .. Briefly, Hek293 cells were transfected with a 0-frame readthrough control pJD2257 plasmid or coronavirus FSE dual-luciferase reporter plasmid using TransIT®-Oligo Transfection Reagent (Mirus Bio). .. At 24 hours post transfection Hek293 cells were washed with 1x PBS and lysed with 1x passive lysis buffer (Promega).

    Article Title: Fucosyltransferase 1 mediates angiogenesis in rheumatoid arthritis
    Article Snippet: .. Upon becoming 70% confluent, 2 μg/ml fut1 sense or antisense ODNs and TransIT-Oligo transfection reagent (Mirus, Madison, WI) was mixed according to manufacturer’s instructions and overlaid on the cells. ..

    Article Title: Dynamic regulation of BDNF gene expression by estradiol and lncRNA HOTAIR.
    Article Snippet: Brain derived neurotrophic factor (BDNF) is a major neurotransmitter that controls growth and maintenance of neurons and its misregulation is linked to neurodegeneration and human diseases.. Estradiol (E2) is well-known to regulate the process of differentiation and plasticity of hippocampal neurons.. Here we examined the mechanisms of BDNF gene regulation under basal conditions and under stimuli such as E2.

    Article Title: Dynamic regulation of BDNF gene expression by estradiol and lncRNA HOTAIR
    Article Snippet: .. Subsequent to this growth, the HT22 cells were transfected with either mouse specific HOTAIR-AS or a scramble antisense oligonucleotide (details in Table 1 ) using the TransIT-Oligo Transfection Reagent (Mirus Bio) ( Ansari et al., 2009 ; Ansari and Mandal, 2010 ). ..

    Article Title: High-throughput immune sequencing
    Article Snippet: Following incubation annealing of the targeted RNAs, the cells can be chemically lysed and the oligo linker-target RNA molecule recovered through its biotin using a streptavidin coated magnetic bead (such as Dynabead M280 form Invitrogen, or other equivalent). .. This oligo linker is then transfected into lymphocytes samples using common transfection technologies such as TransIT®-Oligo Transfection Reagent (Mirus Bio LLC) for example. .. The oligo linker 3′ end can then be used directly for reverse transcription (using superscript III, Invitrogen, or other equivalent reverse transcriptase) of both the heavy and light chains, followed by second strand cDNA synthesis.

    Article Title: CD177-mediated nanoparticle targeting of human and mouse neutrophils
    Article Snippet: Cell were transfected with 25–100 nM siRNA using Dharmafect 1 (Dharmacon) or TransIT-X2 (Mirus Bio LLC) transfection reagents, according to the manufacturer’s protocol. .. For ASO studies, transfections were carried out with no ASO (mock transfected), 50 nM or 100 nM ASO (C5aR1 LNA GapmeR_1) or 100 nM non-targeting control (NTC) using XfectTM RNA transfection reagent (Takara Bio USA) or TransIT®-Oligo transfection reagent (Mirus Bio, LLC) using the manufacturer’s protocol. ..

    Control:

    Article Title: RNA Structural Ensemble Determinants of -1 Programmed Ribosomal Frameshifting Efficiency Across Coronavirus Evolution.
    Article Snippet: .. Briefly, Hek293 cells were transfected with a 0-frame readthrough control pJD2257 plasmid or coronavirus FSE dual-luciferase reporter plasmid using TransIT®-Oligo Transfection Reagent (Mirus Bio). .. At 24 hours post transfection Hek293 cells were washed with 1x PBS and lysed with 1x passive lysis buffer (Promega).

    Article Title: CD177-mediated nanoparticle targeting of human and mouse neutrophils
    Article Snippet: Cell were transfected with 25–100 nM siRNA using Dharmafect 1 (Dharmacon) or TransIT-X2 (Mirus Bio LLC) transfection reagents, according to the manufacturer’s protocol. .. For ASO studies, transfections were carried out with no ASO (mock transfected), 50 nM or 100 nM ASO (C5aR1 LNA GapmeR_1) or 100 nM non-targeting control (NTC) using XfectTM RNA transfection reagent (Takara Bio USA) or TransIT®-Oligo transfection reagent (Mirus Bio, LLC) using the manufacturer’s protocol. ..

    Plasmid Preparation:

    Article Title: RNA Structural Ensemble Determinants of -1 Programmed Ribosomal Frameshifting Efficiency Across Coronavirus Evolution.
    Article Snippet: .. Briefly, Hek293 cells were transfected with a 0-frame readthrough control pJD2257 plasmid or coronavirus FSE dual-luciferase reporter plasmid using TransIT®-Oligo Transfection Reagent (Mirus Bio). .. At 24 hours post transfection Hek293 cells were washed with 1x PBS and lysed with 1x passive lysis buffer (Promega).

    Allele-specific Oligonucleotide:

    Article Title: CD177-mediated nanoparticle targeting of human and mouse neutrophils
    Article Snippet: Cell were transfected with 25–100 nM siRNA using Dharmafect 1 (Dharmacon) or TransIT-X2 (Mirus Bio LLC) transfection reagents, according to the manufacturer’s protocol. .. For ASO studies, transfections were carried out with no ASO (mock transfected), 50 nM or 100 nM ASO (C5aR1 LNA GapmeR_1) or 100 nM non-targeting control (NTC) using XfectTM RNA transfection reagent (Takara Bio USA) or TransIT®-Oligo transfection reagent (Mirus Bio, LLC) using the manufacturer’s protocol. ..



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